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. 2020 Sep 30;10:16167. doi: 10.1038/s41598-020-73285-9

Figure 4.

Figure 4

Quality assessment of purified hAQP10 using a liposome assay and crystallization. (a) Water efflux assay of hAQP10 reconstituted proteoliposomes. Almost identical traces of conventional (blue) and teabag (red) purified samples, using empty liposomes as control (black); (b) glycerol efflux assay of hAQP10 reconstituted proteoliposomes. Almost identical traces of conventional (blue) and teabag (red) purified samples, using empty liposomes as control (black); (c) crystals produced from teabag purified hAQP10, showing rod-shaped crystal morphology; (d) crystals produced from conventionally purified hAQP10, showing needle-shaped crystal morphology; (e) diffraction pattern of conventionally purified hAQP10, showing a diffraction to approximately 2 Å; (f) diffraction pattern of teabag purified hAQP10, showing a diffraction to approximately 2 Å.