Table 1.
Summary of purification results.
| Target | Expression host | Teabag purification resin and method | Yield (mg/L) | Conventional yield (mg/L) | Purification time teabag/conventional (hrs) | QC & application |
|---|---|---|---|---|---|---|
| MraY | E. coli |
IMAC Talon |
0.7 | 1.1 | 16/24 | SEC, enzymatic assay, nanoDSF, ITC |
| ClC-1 | S. cerevisiae |
IMAC Ni–NTA |
0.8 | 1.0 | 8/14 | SEC, negative staining |
| hAQP10 | S. cerevisiae |
IMAC Ni–NTA |
1.2 | 1.8 | 8/14 | SEC, proteoliposomes, crystallization |
| PAR2 | Sf9 insect cells |
IMAC Ni–NTA |
0.6 | 0.7 | 7/10 | SEC |
| KCC2 | Sf9 insect cells | FLAG | 0.5–1.0 | 1.5 | 6/12 | SEC, ligand binding by SPR |
| KCC2 | Sf9 insect cells | FLAG insoluble fraction not removed | 0.5–1.0 | 1.5 | 6/12 | SEC, ligand binding by SPR |