Table 1.
Volumes of reagents for reactions using two different polymerase enzymes combined with three primer-probe sets.
| No. | Reagent | LightCycler Multiplex RNA Virus Master (TIB-Molbiol) | LightCycler Multiplex RNA Virus Master (IDT/Phu Sa) | Invitrogen™ SuperScript™ III One-Step RT-PCR System (TIB-Molbiol) | Invitrogen™ SuperScript™ III One-Step RT-PCR System (IDT/Phu Sa) |
|---|---|---|---|---|---|
| 1 | H2O (RNAse free) | 10.4 μL | 8.4 μL | 5.6 μL | 3.6 μL |
| 2 | Reaction mix | 4.0 μL | 4.0 μL | 12.5 μL | 12.5 μL |
| 3 | Primer E_Sarbeco_F1 | 0.5 μL | 1.0 μL | 0.5 μL | 1.0 μL |
| 4 | Primer E_Sarbeco_R2 | 1.0 μL | 1.0 μL | ||
| 5 | Primer E_Sarbeco_R2 | 0.5 μL | 0.5 μL | ||
| 6 | MgSO4 (50 nM) | 0.4 μL | - | 0.4 μL | 0.4 μL |
| 7 | RT enzyme | 0.1 μL | 0.1 μL | 1.0 μL | 1.0 μL |
| 8 | Template RNA | 5.0 μL | 5.0 μL | 5.0 μL | 5.0 μL |
| Total | 20 μL | 20 μL | 25 μL | 25 μL |