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. 2020 Sep 30;10:16099. doi: 10.1038/s41598-020-73162-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Morphology of cells surface and interior at 48 hpi. (A) With the MOI of 1, virus attachment (arrows) was frequent. (B) Spikes of SARS-CoV-2 particles observed on the cell surface were discernible (MOI of 0.1). Viruses observed on cell surface (cs) exhibited a size between 70 and 85 nm (B, C) at MOIs of 0.01 and 0.1 respectively. Scraping of cells plasma membrane (D) revealed a homogeneous distribution of organelles in mock-infected samples, Infected cells exhibited coated pits vesicles of ≃ 100 nm (arrows) at perinuclear sites (E). A polarized disposal at the infected cells cytosol (F) represented as a condensed area (ca) in the infected ones (MOI of 0.1). (n): nucleus; Bars: (A, C, E) 100 nm; (B) 20 nm; (D) 2 µm; (F) 1 µm.