Fig. 1.

A case study to demonstrate the enzyme mining protocol. A) Quercetin biosynthesis pathway (chalconeflavanone isomerase (CHI)). B) Homologue search using EC number of enzyme in UniProt database. C) Functional ImportantResidue mapping (residue block) to identify true hits (grey colour) from the alignment of homologues and the alignment usedfor Jumpstart PSI-BLAST search. D) FIR mapping (residue block) and phylogenetic analysis performed to identify true enzymehits (grey colour) from our proteome. E) The identified hit were further validated using tissue-based quantification by qRT-PCR(bars) and transcriptome analysis (line).