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. 2020 Sep 19;7:101068. doi: 10.1016/j.mex.2020.101068

Fig. 1.

Fig. 1

hSKP isolation and differentiation to hSKP-HPC. (1) hSKP are isolated from human foreskin. (2) hSKP form spheres in a medium containing EGF and FGF2. (3) Spheres are digested to a 2 dimensional (D) culture, subcultured and cryopreserved. (4) Hepatic differentiation to hSKP-HPC. [Figure abbreviations: BMP: bone morphogenic factor; DEX: dexamethasone; FGF: fibroblast growth factor; HGF: hepatocyte growth factor; hSKP: human skin-derived precursor, hSKP-HPC: human skin-derived precursor hepatocyte progenitor cell, ITS: insulin transferrin selenium; OSM: oncostatin M].