Table 3. Peptide Discovery Results from MSe Data for the Validation of MS-Compatible HDIT101 Surrogate Peptide Candidates.
| number
of b/y ions in MS/MS spectra matched with HDIT101 theoretical peptidesa |
|||||||
|---|---|---|---|---|---|---|---|
| replicate | HC coverage (%) | LC coverage (%) | HC1 | HC2 | HC3 | LC1 | LC2 |
| 1 | 63.9 | 88.6 | 7 | n.d. | 12 | 23 | 6 |
| 75.7 | 84.9 | 3 (n.v.) | n.d. | 7 | 26 | 7 | |
| 2 | 70.6 | 90.0 | 6 | n.d. | 17 | 27 | 12 |
| 66.6 | 89.0 | 4 (n.v.) | 1 (n.v.) | 1 (n.v.) | 16 | 6 | |
| 3 | 67.3 | 83.6 | 1 (n.v.) | n.d. | 7 | 22 | 6 |
| 40.9 | 79.5 | 2 (n.v.) | n.d. | 4 (n.v.) | 12 | 4 (n.v.) | |
| 4 | 64.4 | 90.0 | 5 | n.d. | 2 (n.v.) | 23 | 5 |
| 67.5 | 88.1 | 4 (n.v.) | n.d. | 6 | 12 | 6 | |
| plasma | 54.2 | 60.3 | n.d. | n.d. | 1 (n.v.) | n.d. | 1 (n.v.) |
| 39.2 | 49.3 | n.d. | n.d. | n.d. | n.d. | 1 (n.v.) | |
| suitable | no | no | yes | yes | yes | ||
a
A MS/MS spectrum matching with one HDIT101 theoretical peptide must have ≥ 5 b/y ions to be validated. HC: heavy chain; LC: light chain; n.d.: not detected; n.v.: not validated.