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| mutants | |
|---|---|
| positively charged | N20K+ (denoted as N20K), M21K+ (denoted as M21K), N20K–M21K (denoted as KK-NP), N20K+–M21K+ (denoted as KK-PP), N20R, K22R, N20R–K22R, N20H, M21H, K22H |
| negatively charged | N20E, M21E, and K22E |
| uncharged polar | N20Q |
| hydrophobic | N20V, N20F, M21G, K22G |
| other | N20F–M21L–K22Q (denoted as FLQ) |
