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Physicochemical characterization of DOTAP systems combined with short-chain aminolipids. (A) Hydrodynamic diameter (nm), (B) polydispersity index (PDI), and (C) ζ-potential (mV) for aminolipid-containing DOTAP systems compared to the conventional DOTAP:cholesterol (chol) system at a 1:1 ratio (mol/mol). (D) Transmission electron microscopy (TEM) of DOTAP:chol and (E) AL-A12-containing systems.
