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. 2020 Sep 15;5(38):24724–24732. doi: 10.1021/acsomega.0c03303

Figure 4.

Figure 4

In vitro assessment of transfection efficiency and cytotoxicity of lipoplex systems. (A) Heat map representing the transfection efficiency of aminolipid-containing DOTAP lipoplexes compared to conventional DOTAP:cholesterol (chol) composed lipoplexes. Transfection efficiency was assessed by means of percentage of GFP-positive cells and mean fluorescence intensity (MFI) of GFP expression using flow cytometry. The obtained results were allocated to different groups (color code) based on the quartiles of %GFP-positive cells and MFI. Representative confocal images of HuH7 cells transfected with (B) DOTAP:chol or (C) AL-A12-containing lipoplexes entrapping minicircle DNA coding for GFP (scale bar = 400 μm). (D) Cytotoxicity of aminolipid-containing DOTAP lipoplexes compared to conventional DOTAP:chol lipoplexes by means of lethal concentrations (LC 10/50/90) resulting in 90/50/10% cell viability, respectively.