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. 2020 Sep 18;5(38):24838–24847. doi: 10.1021/acsomega.0c03527

Figure 3.

Figure 3

BBM and SORA synergistically induce HCC cell cycle arrest. (A) Single-agent treatment with BBM (10 μM), SORA (10 μM) alone, or combined treatment with BBM (10 μM) and SORA (10 μM) for 48 h. Cell cycle distribution was assessed using cytometry (Becton Dickinson Fascalibor, BD Biosciences, Franklin Lakes, NJ). For cell cycle distribution, a representative histogram is provided. Data were compiled from three independent experiments and reported in the form of mean ± SD. All of the experiments were performed in triplicate. (B) Western blot analysis of G0/G1 phase-related protein phosphorylated Rb (p-Rb), cyclin D1, Cdk4, and Cdk6. Actin was shown as the control of equal loading. (C) Quantification of the relative expression of cell cycle-related proteins (p-Rb/actin, cyclin D1/actin, Cdk4/actin, and Cdk6/actin) using ImageJ software and analysis with Graphpad prism 7. **P < 0.01 compared to the negative control.