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. 2020 Sep 18;5(38):24838–24847. doi: 10.1021/acsomega.0c03527

Figure 4.

Figure 4

BBM and SORA synergistically induce HCC cell apoptosis. (A) Single-agent treatment with BBM (10 μM), SORA (10 μM) alone, or combined treatment with BBM (10 μM) and SORA (10 μM) for 48 h. Apoptosis was analyzed using Annexin V/propidium iodide (PI) staining. Data were compiled from three independent experiments and reported in the form of mean ± SD. All of the experiments were performed in triplicate. (B) Extraction of total protein and detection of the expression of cleaved PARP, cleaved caspase 3, and Bcl-2 using western blotting. Actin was shown as the control of equal loading. (C) Quantification of the relative expression of apoptosis-related proteins (cleaved PARP/actin, cleaved caspase 3/actin, and Bcl-2/actin) using ImageJ software and analysis with Graphpad prism 7. **P < 0.01 compared to the negative control.