FIGURE 4.

(A) miR-21 putative binding sites in SOX6 3′-UTR. Pairing schematic of SOX6 3′-UTR and miR-21; blue text indicates wild-type sites, and red text indicates mutated sites in the psiCHECK-2 reporter plasmid. The constructs were cotransfected with miR-21 mimics (or negative control) into 293T cells and dual-luciferase assays were performed 48h after transfection. (Data represent mean ± SD, **P < 0.01, ***P < 0.001.) (B) miR-21 putative binding sites in MSTRG.223165 3′-UTR. Pairing schematic of MSTRG.223165 3′-UTR and miR-21; blue text indicates wild-type sites, and red text indicates mutated sites in the psiCHECK-2 reporter plasmid. The constructs were cotransfected with miR-21 mimics (or negative control) into 293T cells and dual-luciferase assays were performed 48 h after transfection. (Data represent mean ± SD, **P < 0.01, ***P < 0.001.)