Table 2.

Histopathological analysis of the two-stage bIRI model

Compartment	Parameter	Two-Stage bIRI 0-min Group	Two-Stage bIRI 15-min Group	Two-Stage bIRI 18-min Group	Two-Stage bIRI 21-min Group	Two-Stage bIRI 24-min Group
Tubule and interstitium	Tubular atrophy (0–3)	0	0	0	2	2
	Interstitial fibrosis (0–3)	0	0	0	1	2
	Inflammatory cell invasion (0–3)	0	0	0	1	3
Glomeruli	Glomerulosclerosis index (0–4)	0 ± 0	0 ± 0	0.02 ± 0.01	2.39 ± 0.23	3.02 ± 0.20
	Collapse of glomerular tufts (present/absent)	0/100	0/100	0/100	16/84	56/44
	Dilatation of Bowman’s capsules (present/absent)	0/100	0/100	1/99	21/79	62/38

Tubular atrophy, interstitial fibrosis, inflammatory cell infiltration, glomerulosclerosis, collapse of glomerular tufts, and dilated Bowman’s capsules were observed in the two-stage bilateral ischemia-reperfusion injury (bIRI) 21-min and two-stage bIRI 24-min groups but not in the two-stage bIRI 15-min, two-stage bIRI 18-min, or two-stage bIRI 0-min groups. Tubular atrophy, interstitial fibrosis, and inflammatory cell invasion were scored on a scale of 0−3 according to the following criteria: 0, affecting 0–5% of the renal area; 1, affecting 6–25% of the renal area; 2, affecting 26–50% of the renal area; and 3, affecting >50% of the renal area (n = 10 fields/mouse, 9 or 10 mice/group). The glomerulosclerosis index was scored on a scale of 0−4 using the following criteria: 0, normal glomeruli; 1, 1–25% of the sclerotic area; 2, 26–50% of the sclerotic area; 3, 51–75% of the sclerotic area; and 4, 76–100% of the sclerotic area; collapse of glomerular tufts as well as dilatation of Bowman’s capsules were graded by their presence or absence (n = 10 glomeruli/mouse, 10 mice/group).