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. 2020 Oct 1;20:475. doi: 10.1186/s12935-020-01573-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — miR-138-5p targeted NFIB. a Target genes of miR-138-5p were predicted by three databases, and NFIB was screened out based on biological function. b Relative NFIB mRNA level negatively correlated with relative miR-138-5p level (r = − 0.242, p < 0.05). c Relative NFIB protein level negatively correlated with relative miR-138-5p level (r = − 0.249, p < 0.05). d NFIB protein expression in stable cell lines. e In HCT116 cell lines, LV-mimics group was lower in the expression of NFIB protein than LV-mNC group (p < 0.001). In DLD1 cell lines, LV-inhibitors group was higher in the expression of NFIB protein than LV-INC group (p < 0.001). f In HCT116 cell lines, LV-mimics group was lower in the expression of NFIB mRNA than LV-mNC group (p < 0.001). In DLD1 cell lines, LV-inhibitors group was higher in the expression of NFIB mRNA than LV-INC group (p < 0.001). g The wild-type and mutant plasmids of NFIB mRNA 3 UTR were constructed on the potential binding sites of miR-138-5p and NFIB mRNA. h Double luciferase assay verified that miR-138-5p banded with wild type NFIB mRNA 3 UTR instead of mutant type (p < 0.001). i The NFIB siRNA with the best interference effect sequences was screened for subsequent experiments