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. 2020 Oct 1;20:475. doi: 10.1186/s12935-020-01573-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — NFIB activated Snail1. a NFIB mRNA and Snail1 mRNA expression had positive correlation in 100 colorectal cancer tissues (r = 0.31, p < 0.001). b In GEPIA database, NFIB mRNA and Snail1 mRNA expression were positively correlated (r = 0.646, p < 0.001). c Expression of Snail1 and NFIB after transfected with NFIB siRNA. d After transfected with NFIB siRNA, the relative protein level of Snail1 were decreased (p < 0.01). e After transfected with NFIB siRNA, the relative mRNA level of Snail1 were decreased (p < 0.01). f CHIP experiment verified that transcription factor NFIB could bind to the promoter region of Snail1 gene. g–i. According to the binding sites predicted by the database, wild-type and mutant plasmids of the Snail1 promoter region were constructed. Double luciferase assay experiment verified that NFIB binded to the wild-type promoter region of Snail1, instead of the mutant type. j–k Expression of Snail1 was detected after co-transfected NFIB siRNA into stable cell lines. The difference was statistically significant between NFIB siRNA-LV inhibitor + LV INC− group and NFIB siRNA-LV inhibitor-LV INC + group (p < 0.001) The difference was not statistically significant between NFIB siRNA + LV inhibitor-LV INC + group and NFIB siRNA + LV inhibitor + LV INC− group (p > 0.05)