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. 2020 Oct 1;19:185. doi: 10.1186/s12934-020-01446-6

Fig. 2.

Fig. 2

Flow cytometry analysis of spores of strains carrying the cotB::tetC or cotC::tetC gene fusion. Spores were produced at 25, 37 or 42 °C and reacted with anti-TTFC antibody, then with FITC-conjugated secondary antibody. The analysis was then performed on the entire spore population (ungated). In each panel is indicated the percentage of positive events above the fluorescent intensity threshold of 1 × 103 (red line). As control experiment spores of an isogenic wild type strain (PY79) not containing any gene fusion were analyzed