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. 2020 Sep 19;5(38):24927–24934. doi: 10.1021/acsomega.0c03750

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SDS-PAGE analysis of monomeric scFv-SpyCatcher (1), scFv-SpyCatcher-SpyTag002-oligonucleotide (2), and dimeric scFv-AP-SpyCatcher (3,5), scFv-AP-SpyCatcher-SpyTag002-oligonucleotide (4,6). M, molecular weight marker; lane 1, anti-MC-LR scFv-SpyCatcher; lane 2, anti-MC-LR scFv-SpyCatcher-SpyTag002-oligonucleotide; lane 3, anti-MC-LR scFv-AP-SpyCatcher; lane 4, anti-MC-LR scFv-AP-SpyCatcher-SpyTag002-oligonucleotide; lane 5, anti-E2 scFv-AP-SpyCatcher; lane 6, anti-E2 scFv-AP-SpyCatcher-SpyTag002-oligonucleotide. Abbreviations: scFv: single-chain variable fragment; MC-LR: microcystin-LR; AP: alkaline phosphatase; E2: 17β-estradiol.