. 2020 Sep 17;11:572314. doi: 10.3389/fmicb.2020.572314

TABLE 4.

Changes in fungal metabolic diversity in soil contaminated with crude oil after long-term natural bioremediation as evaluated by: Shannon’s general diversity index (H’), substrate richness (R), substrate evenness (E), and average well-color development (AWCD₅₉₀) obtained in the Biolog FFPlates incubated for 120 h and ITS next generation sequencing (total richness indicators calculated on the basis of all sequences = real diversity).

Sample	H’	R	E	AWCD₅₉₀_{_}

	Biolog FFPlate
OWP1	4.45^a	95^a	0.994^a	0.775^b
OWP2	4.52^a	94^a	0.982^a	0.681^b
OWP3	4.61^a	94^a	0.986	0.895^ab
OW1	4.01^b	92^ab	0.951^b	0.972^a
OW2	4.12^b	90^b	0.942^b	0.998^a
OW3	4.21^b	91^b	0.956^b	0.979^a

	ITS NGS
	H’	Chao1	Simpson	Fisher

OWP1	4.86^a	512^b	49.53^b	88.70^a
OWP2	4.91^a	445^b	64.93^a	76.71^b
OWP3	4.65^ab	447^b	44.31^b	68.87^b
OW1	4.52^b	604^a	27.48^c	92.56^a
OW2	4.84^a	318^c	63.83^a	46.43^c
OW3	4.41^b	585^a	27.95^c	89.79^a

Treatment means separated by different letters (a,b,c,d) are significantly different (Tukey’s mean separation test, p < 0.05).