Figure 1.

A and B, Results of Sanger sequencing of CYP11B2-expressing tumor region for both aldosterone-producing adenomas (APAs) harboring somatic CLCN2 mutations. The top row of nucleotides denotes the nucleotide trace in the matched normal adjacent adrenal tissue obtained by Sanger sequencing. The variant in APA_UM14 (c.64-2_74del) suggests a splice site deletion mutation. C, Quantitative reverse transcriptase–polymerase chain reaction analysis for expression of CLCN2 messenger RNA levels in tumor (T) vs their respective adjacent normal tissue (N). Results are indicated as mean. Red and blue symbols indicate the N-T pair for APA_UM14 and APA_UM16, respectively.