Table 2.
Characteristics of the XAC1051-2qPCR assay performed using three different devices
| Plant matrix | StepOnePlus | QS | LC480 | |
|---|---|---|---|---|
| qPCR Efficiency | lemon | 93% | 94% | 97% |
| orange | 102% | 103% | 106% | |
| R2 | lemon | 0.992 | 0.981 | 0.984 |
| orange | 0.988 | 0.986 | 0.987 | |
| Intra-assay variation Cv range (median) |
0.07–2.48% (0.34) |
0.13–4.6% (0.60) |
0.14–3.6% (0.81) |
|
| Inter-assay variation Cv range (median) |
0.49–2.5% (1.50) |
0.55–3% (1.30) |
0.84–2.4% (1.50) |
|
| Cut-off values | 35.9 | 38.43 | 38.89 | |
|
LOD95% (CI) |
2.90 (2.76–3.04) |
3.04 (2.96–3.11) |
3.09 (2.92–3.25) |
|
| Vic Ct range | 25.8–31.2 | 22.3–27.9 | 23.4–29.7 |