Table 3.
Comparison of the specificity of several PCR and real-time qPCR protocols
| Assay | X. citri pv. citri | X. citri pv. aurantifolii (n = 5) | X. euvesicatoria pv. citrumelonis (n = 2) | Other X. citria pathovars (n = 9) | Other Xanthomonasa species (n = 11) | Saprophytic xanthomonadsb (n = 15) | |||
|---|---|---|---|---|---|---|---|---|---|
| A (n = 63) | A* (n = 11) | Aw (n = 4) | |||||||
| PCR | Jpth1/2 | 62c | 11 | 4 | 5 | 0 | 7 | 1 | 0 |
| PCR | VM3/4 | 63 | 11 | 4 | 5 | 0 | 7 | 1 | 0 |
| PCR | XACF/R | 63 | 11 | 4 | 0 | 0 | 4 | 0 | 0 |
| PCR | XCF/R | 63 | 11 | 4 | 0 | 0 | 9 | 0 | 0 |
| PCR | XAC1051-F/R | 63 | 11 | 4 | 0 | 0 | 0 | 0 | 0 |
| qPCR | XAC1051-2qPCR | 63 | 11 | 4 | 0 | 0 | 1 | 0 | 0 |
| qPCR | J-Taqpth-qPCR | 63 | 11 | 4 | 0 | 0 | 3 | 0 | 0 |
| qPCR | VM-Syb-qPCR | 63 | 11 | 4 | 5 | 0 | 7 | 1 | 0 |
a Isolated from Citrus spp. but not pathogenic to Citrus spp.
b Isolated from Citrus spp.
c Number of positive samples