Fig. 4. Identification and structural determination of aldehyde-alcohol dehydrogenase (AdhE).
(A) A negative-stain EM micrograph of the fraction containing the spiral architecture. Scale bar = 50 nm. (B) SDS-PAGE analysis of the fraction containing the spiral architecture (left panel). The top five abundant proteins from mass spectrometry identification are listed (right panel). (C) SDS-PAGE analysis of the purified recombinant E. coli AdhE protein. (D) A negative-stain EM micrograph of the recombinant E. coli AdhE protein showing spiral architectures. Scale bar = 50 nm. (E) Comparison among AdhE spiral architectures from negative-stain micrographs from cell lysates (left panel), recombinant protein (middle panel), and the cryo-EM structure (right panel; PDB ID: 6AHC).