Skip to main content
. 2020 Oct 1;34(19-20):1392–1405. doi: 10.1101/gad.328575.119

Figure 6.

Figure 6.

Alt-NHEJ competes with BIR at telomeric DSBs. (A) Quantification of q arm fragile telomeres detected by FISH in BlmF/F MEFs ± Cre (96 h) treated with or without 2 μM olaparib for 20 h. All P-values were derived from two-tailed paired t-test. (*) P ≤ 0.05. (B) Western blot analysis of Ligase 3 in BlmF/F MEFs with γTubulin as the loading control. (C) Quantification of q arm fragile telomeres detected by FISH in BlmF/F MEFs ± Cre (96 h) with CRISPR/Cas9 targeting of Luc or Xpf and/or shRNAs targeting Luc or LIG3. (D) Quantification of leading- and lagging-end q arm fragile telomeres detected by CO-FISH in BlmF/F MEFs ± Cre (96 h) treated with or without 2 μM olaparib for 20 h. (E) Quantification of leading- and lagging-end q arm fragile telomeres detected by CO-FISH in BlmF/F MEFs ± Cre (96 h) with CRISPR/Cas9 targeting of Luc or Xpf and/or shRNAs targeting Luc or LIG3. For all fragile telomere analyses in this figure, the data are means ± SD of three independent experiments of ∼2000 telomeres analyzed per experiment. All P-values in this figure except for panel A were derived from two-tailed unpaired t-test. (***) P ≤ 0.001, (**) P ≤ 0.01, (*) P ≤ 0.05. (F) Model showing how alt-NHEJ and BIR act on telomeric DSBs in Blm-deficient cells and the effect of alt-NHEJ on the detection of fragile telomeres.