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. 2020 Oct 1;34(19-20):1345–1358. doi: 10.1101/gad.340661.120

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© 2020 King et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 5. — Transcriptional activity of Yap/Taz is essential for macropinocytosis and cell growth requiring the catabolism of extracellular proteins. (A) Western blot of FLAG immunoprecipitates and input lysates from Yap/Taz dKO KRPC cells transduced with retroviruses containing a doxycycline-inducible, FLAG-tagged, Taz cDNA (either wild type, S89A, or S51A mutant) or empty vector. Cells were cultured for 24 h in 100 ng/mL doxycycline prior to harvest. (B) Relative expression of Ankrd1 and Axl mRNA in WT KRPC or Yap/Taz dKO cells transduced with retroviruses containing a doxycycline-inducible Yap or Taz cDNA (wild type or with the indicated mutation) after culture for 24 h in 100 ng/mL doxycycline. Bars represent mean ± SD of three biological replicates. (C) Uptake of fluorescent dextran (left panel) and albumin (right panel) in Yap/Taz dKO cells transduced with retroviruses containing a doxycycline-inducible Yap or Taz cDNA after culture for 24 h in 100 ng/mL doxycycline. Bars represents mean ± SEM fluorescence intensity per cell, across 12 fields of view. (D) Growth of WT KRPC or Yap/Taz dKO cells transduced with retroviruses containing a doxycycline-inducible Yap or Taz cDNA after 96 h in leucine-free medium + 3% BSA and 100 ng/mL doxycycline. Bars represent mean ± s.d. of three technical replicates. (***) P < 0.0001, (**) P < 0.005, (*) P < 0.05 one-way ANOVA, for C and D.