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. 2020 Sep 17;11:1083. doi: 10.3389/fphar.2020.01083

FIGURE 5.

FIGURE 5

Effects of LS-102 on the mitochondrial dysfunction induced by H/R in H9c2 cells. (A) Effect of LS-102 on mitochondrial viability, the content of ATP, and SOD activity. (B) The Seahorse XFp Extracellular Flux Analyzer was used to evaluate the effect of LS-102 on oxygen consumption rate (OCR). (C) Representative images of JC-1 fluorescent dye stained the mitochondrial membrane potential captured by confocal microscopy with ×40 objective (Olympus). Using the fluorescence microplate assay to calculate the red/green ratio. (D) The protein of cytochrome c was detected by Western blot. (E) Representative images of Fluo-4AM stained calcium captured by confocal microscopy with ×20 objective (Olympus). The relative mean of the calcium fluorescence intensity was analyzed by flow cytometry. (F) The content of the ROS labeled H2DCFDA probe was analyzed by flow cytometry. The values were the means ± SEM of three or four independent experiments. ^^P < 0.01, ^^^P < 0.001 vs. Normal group. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. H/R group.