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. 2020 Oct 1;202(7):1013–1023. doi: 10.1164/rccm.201911-2151OC

Figure 1.

Figure 1.

Age-associated B cell (ABC)-like cells are enriched in the peripheral blood mononuclear cells (PBMCs) and BAL of patients with sarcoidosis and are reduced in percentage in patients on therapy. PBMCs and BAL cells were obtained from healthy subjects (HSs), sarcoidosis patients not on treatment (SarcPs), and sarcoidosis patients on treatment (SarcPTs) as described in Methods. The treatment protocols and data for the patients are listed in Table 1. Cells were stained as described in Methods, and staining was detected on a CyAn (Beckman Coulter) instrument. Data were analyzed using FlowJo software (Tree Star). The percentage of PBMC B cells from HSs and SarcPs that were (A) ABC-like, (B) ABC-like T-betneg-lo, and (C) ABC-like T-bethi are shown. The results in each panel were analyzed using Mann-Whitney tests. (D–F) Data are as described for A–C, except each panel compares data for each cell type for SarcPs and SarcPTs. (G–I) Data are as described for A–C, except each panel contains data for the indicated cell types from the B cells in PBMCs of SarcPs and B cells in BAL from the same patients. *P < 0.05, **P < 0.01, and ***P < 0.001. PBL = peripheral blood lymphocytes.