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A549/ABCA3−/− cells that express p.E292V or p.L101P have decreased Top-Fluor-phosphatidylcholine (TopF-PC)–labeled liposome uptake compared with A549/ABCA3−/− cells that express ABCA3 WT. We used (A) single x-y–plane imaging and (B) Pearson correlation to measure immunofluorescence colocalization of ABCA3 WT or mutants (mCherry) with TopF-PC (green). Experiments were performed three times with similar results. *P < 0.001. Scale bar, 10 μm.
