. 2020 Oct 1;6(10):e05143. doi: 10.1016/j.heliyon.2020.e05143

Table 1.

Real-Time PCR primer sequences and amplification settings.

Genes	Accession number		Sequence (5′-3′)	Annealing temperature (C°)	Size (bp)
ACE2	NM_001371415	Fw	GGTGGGAGATGAAGCGAGAG	60	150
ACE2	NM_001371415	Rev	CTTGAAACTGGAATTGGTAAAGGGT	60	150
TMPRSS2	NM_005656	Fw	GGGACATGGGCTATAAGAAT	53	137
TMPRSS2	NM_005656	Rev	ACAGGCATCACTGTGGTAC	53	137
PCSK3	NM_002569	Fw	GCATTGGAGGGGTGCGCAT	61	106
PCSK3	NM_002569	Rev	CCCCAGCTGGCACTGTAGAT	61	106
EMILIN1	NM_007046	Fw	CCAAAGCATCATGTACCGCC	58	110
EMILIN1	NM_007046	Rev	CACAGTCATCGCCCCCATAA	58	110
MMRN1	NM_007351	Fw	TAGCTCCAGATTTTTCCAAAGGA	57	112
MMRN1	NM_007351	Rev	TCCAAGTTATTAAACAGGATAGGAC	57	112
EMILIN2	NM_032048	Fw	TGTCTGGGCGGGGTCTG	62	102
EMILIN2	NM_032048	Rev	GTGACTTCGGGTATCCTGGTG	62	102
MMRN2	NM_024756	Fw	CCAGGCTTCCAGTACTAGCC	59	111
MMRN2	NM_024756	Rev	GGGCACCAGTTACGTCCTAC	59	111
DPP4	NM_001935	Fw	GAAAGGTGTCAGTACTATTCTGTGT	59	130
DPP4	NM_001935	Rev	CCAGGACTCTCAGCCCTTTATC	59	130
GAPDH	NM_002046	Fw	AAGGTCGGAGTCAACGGATTT	59	100
GAPDH	NM_002046	Rev	TGAAGGGGTCATTGATGGCA	59	100
RPLP0	NM_001002	Fw	ACCCAGCTCTGGAGAAACT	57	198
RPLP0	NM_001002	Rev	AAAAGGAGGTCTTCTCGGG	57	198
ACTB	NM_001101	Fw	ATTGCCGACAGGATGCAGAA	59	150
ACTB	NM_001101	Rev	GCTGATCCACATCTGCTGGAA	59	150

ACE2 (Angiotensin-converting enzyme 2), TMPRSS2 (Transmembrane protease serine 2), PCSK3 (proprotein convertase subtilisin/kexin 3), EMILIN1 (elastin microfibrils interface located protein-1), MMRN1 (multimerin-1), EMILIN2 (elastin microfibrils interface located protein-2), MMRN2 (multimerin-2), DPP4 (Dipeptidyl peptidase-4), GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase), RPLP0 (ribosomal protein, large, P0), ACTB (β-Actin). Fw (Forward), Rev (Reverse). PCR (Polymerase Chain Reaction).