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Effect of fixation on infectious virus in cells. A, TCID50 assay comparing untreated infected cells with fixed infected cells. Lower limit of detection was 0.5 TCID50 (log10)/mL (n = 3; mean ± SEM). B, The proportion of inactivation assays with CPE. Abbreviations: CPE, cytopathic effect; SEM, standard error of the mean; TCID50, 50% tissue culture infectious dose.
