Fig. 4.
Fasting serum NEFA, basal glycerol release, and lipolytic proteins are reduced in SK1fatKO mice. A: Serum NEFAs were measured from mice fasted for 6 h. B: gWAT explants were incubated in fatty acid-free medium for 48 h and medium glycerol was measured. Medium glycerol was normalized to the weight of tissue utilized in each well. C: Western blot protein analysis of lipolytic proteins (ATGL, CGI-58, FABP4) from gWAT. Protein loading was normalized by stain-free total loading, as well as vinculin. D: Band intensities were quantified by densitometry using ImageJ and graphed separated by sex (males on left, females on right) showing mean ± SEM.