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. 2020 Sep 21;16(9):e1008834. doi: 10.1371/journal.ppat.1008834

Fig 6. ZNF304 mediates H3K27me3 and H2AK119Ub histone marks on the HIV promoter through TRIM28 and PRC1/2 to silence HIV gene transcription A, B and D.

Fig 6

ChIP material was isolated from control or ZNF304-depleted cells. Immuno-precipitation was conducted with H3K27me3 (A), H2AK119ub (B), or Pol II (D). In all experiments, non-specific rabbit IgG was used as the control. qPCR on IP samples was conducted with primers located on the HIV promoter, and signals are presented as a percentage of input. Error bars represent means ± SD of three independent qPCR reactions. Asterisks indicate different levels of statistical significance as calculated by a two-tailed Student’s t test (** p≤0.01). C. TRIM28 associates with EZH2 in cells–HEK293T cells expressing HA-TRIM28 were subjected to immunoprecipitation with HA IgG or control IgG. IP samples were then subjected to SDS-PAGE and western blot with HA IgG. Input samples represent 5% of the total cell lysate.