Freshly harvested conidia from the AF293 isolate were delivered via aerosolization (12 mL, 1 X 109 conidia/mL) to immuno-competent wild type and Nlrx1-/- mice. Recruited immune cell populations in (AB) bronchoalveolar lavage fluid BALF and (C-J) pulmonary tissue by wild type and Nlrx1-/- mice on day 1 and 3 post inoculation were determined by flow cytometry. Asterisk denotes statistical significance, P < 0.05 Mann-Whitney U test. Error bars indicate standard deviation. N = 8–10 per experimental group. AM, alveolar macrophages. IM, interstitial macrophages. DC, dendritic cells. pDC, plasmocytoid dendritic cells. moDC, monocytoid dendritic cells. cDC, conventional dendritic cells. NK, natural killer cells.