Fig 8. In situ hybridization assay utilizing RNAscope detects RNA for Nlgn2 and Ntrk3 within P4 cochlear whole mounts.

(A) Cochlear whole mounts treated with negative control sense probe for Nlgn2 show no signal. Hair cells were immunostained with anti-Myo7a antibody (green). (B) Nlgn2 is expressed in the region of spiral ganglion neurons (red), but not hair cells (green). A magnified view of the SGN region (last row) identifies individual RNA molecules of Nlgn2 as red dots. (C) Ntrk3 is expressed in the SGN region (red), but not hair cells (H, green). A magnified view of the SGN region (last row) reveals abundant Ntrk3 expression. Representative pictures based on 4 different animals.