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. 2020 Oct 1;16(10):e1008881. doi: 10.1371/journal.ppat.1008881

Fig 4. T. denticola, P. gingivalis and F. nucleatum promote orasphere formation of OSCC cells and nisin inhibits that process.

Fig 4

Cells (UM-SCC-14A and HSC-3) were challenged with control medium or media containing different MOIs of T. denticola, P. gingivalis or F. nucleatum for 2 h then treated with nisin (50 μg/ml) under suspension conditions for 24 h. (A-F) Representative phase contrast images are shown, and graphs show the fold change in orasphere formation after 24 h (magnification 100x). Data represent mean ± SD from three independent experiments. *Comparison between groups relative to their media controls *p≤0.05; #Comparison between groups relative to their matching concentrations of pathogens alone #p≤0.05. Comparison between groups relative to their media control p≤0.05.