Fig 2. Mitochondrial bioenergetics in EDL PmFB.

ADP-stimulated respiration was initially supported by complex I (NADH from 5mM pyruvate/4mM malate) in the presence of creatine with an ADP titration of 25μM, 100μM, 500μM and 5mM concentrations (n = 12) (A) directly followed by complex II (FADH₂ from 20mM succinate, Succ) (n = 11–12) (B). Mitochondrial H₂O₂ emission was stimulated at complex I (NADH from 10mM pyruvate/4mM malate) (n = 12) (C) followed by an ADP titration and expressed relative to maximal H₂O₂ emission in response to pyruvate/malate before ADP was titrated (% of State II) (n = 11–12) (D). Calcium retention capacity (n = 10) (E). Results are reported as mean ± SEM, (*p<0.05 vs wildtype).