Fig 1. Genomic information hierarchy encompassing individual, population and community levels of cellular organization.

(a) Building on the BioCyc curation-tiered structure of Pathway/Genome Databases (PGDBs) constructed from organismal genomes, two additional data structures are resolved from single-cell and plurality sequencing methods to define a 4 tiered hierarchy (T1-4) in descending order of manual curation and functional validation. (b) Completion scales for organismal genomes, single-cell amplified gemomes (SAGs) and metagenome assembled genomes (MAGs) within the 4 tiered information hierarchy. Genome completion will have a direct effect on metabolic inference outcomes with incomplete organismal genomes, SAGs or MAGS resolving fewer metabolic interactions.