Table 1.

The Seeding Density of Feeder Cells and Lung Stem Cells in Different Cell Culture Vessels

Cell Culture Vessel	Approx. Growth Area (cm2)	Recommended Culture Volume (mL)	Recommended No. of Feeder Cells	Recommended Seeding Density of Stem Cells
6 well	9.5	2	1,500,000–1,800,000	50,000–100,000
12 well	3.8	1	600,000–720,000	15,000–30,000
24 well	1.9	0.5	300,000–400,000	8,000–15,000
48 well	0.95	0.25	120,000–150,000	5,000–7,000
96 well	0.32	0.125	50,000–60,000	1,000–3,000
384 well	0.056	0.05	10,000–15,000	500–1,000
100 mm dish	55	12	9,000,000–11,000,000	500,000–1,000,000
150 mm dish	152	30	25,000,000–30,000,000	1,200,000–2,500,000
12 well Transwell	1.12	well 1.5, insert 0.5	800,000–1,000,000	400–600,000
24 well Transwell	0.33	well 0.7, insert 0.2	300,000–400,000	200–300,000