Tissue Processing Buffer

Reagent	Amount	Final Concentration
F12	914 mL	n/a
FBS	50 mL	5%
Penicillin-streptomycin	10 mL	1%
Gentamycin	10 mL	1%
HEPES	15 mL	1.5%
Fungizone	1 mL	0.1%