REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal CC10 Dilution factor: 1:100	Santa Cruz	Cat# sc-130411; RRID: AB_2183388
Rabbit polyclonal SFTPC Dilution factor: 1:100	Santa Cruz	Cat# sc-13979; RRID: AB_2185502
Rabbit polyclonal MUC5AC Dilution factor: 1:100	Santa Cruz	Cat# sc-20118; RRID: AB_2146854
Rabbit polyclonal AQP4 Dilution factor: 1:200	Sigma-Aldrich	Cat# HPA014784; RRID: AB_1844967
Rabbit polyclonal SFTPB Dilution factor: 1:200	Atlas Antibodies	Cat# HPA034820; RRID: AB_2674347
Mouse monoclonal acetylated tubulin Dilution factor: 1:500	Sigma-Aldrich	Cat# T7451; RRID: AB_609894
Mouse monoclonal p63 Dilution factor: 1:200	Abcam	Cat# ab735; RRID: AB_305870
Biological Samples
Human Lung samples	UCONN Health; UTHSC; UIHC	NA
Chemicals, Peptides, and Recombinant Proteins
DMEM without L-glutamine, without sodium pyruvate	Invitrogen	Cat# 11960-044
F12	Life Technologies	Cat# 11765-054
HEPES	Life Technologies	Cat# 15630-080
Gentamycin	Life Technologies	Cat# 15710-064
Fungizone	Life Technologies	Cat# 15290018
Bovine Calf Serum (BCS)	HyClone	Cat# SH130072.03
Fetal Bovine Serum (FBS)	HyClone	Cat# SH30396.03
Penicillin-streptomycin	Invitrogen	Cat# 15140-122
L-glutamine	Invitrogen	Cat# 25030-081
0.05% Trypsin-EDTA	Invitrogen	Cat# 25300-062
DPBS, no calcium, no magnesium	Invitrogen	Cat# 14190-144
Dimethyl sulfoxide (DMSO)	Sigma-Aldrich	Cat# 41640
Feeder removal beads	Miltenyi Biotec	Cat#130-095-531
TrypLE™ Express Enzyme (1×), phenol red	Thermo Fisher	Cat#12605036
Trypan blue solution	Corning	Cat# 25-900-CI
Corning™ Matrigel™ GFR Membrane Matrix	Fisher Scienitific	Cat# CB-40230
Collagenase Type IV	Life Technologies	Cat# 17104-019
StemECHO100	Nüwa Medical Systems, Inc.	http://www.stemecho.com
StemECHO™PU Expansion medium	Nüwa Medical Systems, Inc.	http://www.stemecho.com
PneumaCult-ALI Medium	STEMCELL Technologies	Cat# 05001
Experimental Models: Cell Lines
3T3-J2	Kerafast	Cat# EF3003
Experimental Models: Organisms/Strains
Mouse: NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ	The Jackson Laboratory	Stock No:005557 | NSG
Software and Algorithms
SH800S Cell Sorter Software version 2.1	SH800S Cell Sorter	https://www.sonybiotechnology.com/us/instruments/sh800s-cell-sorter/software/
Other
Inverted microscope	Nikon	Eclipse Ts2
CO2 incubator	Panasonic	Cat# KM-CC17RU2
Tissue culture biological safety cabinet	NuAire LabGard ES	Cat# NU-437
Student Dumont no. 5 forceps	Interfocus	Cat# 91150-20
Fine scissors straight large loops 10 cm	Interfocus	Cat# 14040-10
Pasteur pipettes	Scientific Laboratory Supplies	Cat# PIP4172
Sterile disposable scalpels	Dynarex	Cat# 4122
6 Well Falcon™ Polystyrene Microplates	Fisher Scientific	Cat# 08-772-1B
12 Well Falcon™ Polystyrene Microplates	Fisher Scientific	Cat# 08-772-29
24 Well Falcon™ Polystyrene Microplates	Fisher Scientific	Cat# 08-772-1
Nunc™ 384-Well, Nunclon Delta-Treated, Flat-Bottom Microplate	Fisher Scientific	Cat# 12-565-342
Nalgene™ Rapid-Flow™ Sterile Disposable Filter Units with PES Membrane	Fisher Scientific	Cat# 09-741-03
Steriflip 50 mL filtration units	Millipore	Cat# C3238
100 μm cell strainer	Falcon	Cat# 08-771-19
Pre-separation filters (30 μm)	Miltenyi Biotec	Cat# 130-041-407
QuadroMACS Starting Kit (LS)	Miltenyi Biotec	Cat# 130-091-051
Transwell with 0.4um pore polyester membrane insert	Fisher Scientific	Cat# 07-200-154
Cell Sorter	Sony	Cat# SH800S
X-ray irradiation machine	RadSource	Cat# RS1800
Mini shaking incubator	WISBiomed	Cat# BS-ICH1
Centrifuge	Eppendorf	Cat# 5810 R
Hemocytometer	Daigger	Cat# EF16034F
Cool Cell Freezing Container	Corning	Cat# 432008
-80°C freezer	VWR	Cat# 76307-942
Liquid nitrogen tank	VWR	Cat# 10027-532