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Effect of addition of zinc and chitosan on thermal stability and association state of insulin determined using nano differential scanning calorimetry. [Insulin: 1 mg/mL; Zinc acetate: 1:5 molar ratio of insulin hexamer to zinc ions; Chitosan: 5 moles of CS monomer unit per mole of insulin monomer; in phosphate buffered saline (10 mM, pH 7.4)]
