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. 2020 Sep 30;5(5):e00717-20. doi: 10.1128/mSphere.00717-20

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Copyright © 2020 Blanco et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — Fitness determination in MIEM and LB growth media. Exponential growth rates were determined by OD₆₀₀ measurements over time for clones isolated from LL-37, PR-39, and colistin-evolved populations in MIEM (A) or LB (B) media. Relative growth rates were calculated using the parental strain D457 as a reference (dotted line). Error bars represent standard deviation for five independent replicates. Statistical significance relative to D457 was assessed by one-way analysis of variance (ANOVA) test (**, P > 0.0001; *, P > 0.001).