Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent Mus musculus | C57BL/6J | Jackson Laboratory | Stock #: 000664 RRID:MGI:5657312 |
WT mouse |
| Genetic reagent Mus musculus | B6.129S6(Cg)-Rhotm1.1Kpal/J | Jackson Laboratory | Stock #: 017628 RRID:IMSR_JAX:017628 | RhoP23H/P23H mouse |
| Genetic reagent Mus musculus | Gnat2tm1(KOMP)Vlcg | Marie E. Burns (University of California, Davis) | Original reference: doi:10.1016/j.exer.2018.02.024 | Gnat2-/- mouse |
| Antibody | Anti-PKCα (rabbit monoclonal) | Abcam | Cat. #: ab32376 RRID:AB_777294 | IHC (1:2000) |
| Antibody | Anti-Calbindin (mouse monoclonal) | Abcam | Cat. #: ab75524 RRID:AB_1310017 | IHC (1:1000) |
| Antibody | Anti-vGlut1 (guinea pig polyclonal) | Millipore | Cat. #: ab5905 RRID:AB_2301751 | IHC (1:1000) |
| Antibody | Anti-mGluR6 (sheep) |
Jeannie Chen (University of Southern California) | Originally developed by Kirill Martemyanov lab, doi:10.1523/JNEUROSCI.1367–09.2009 | IHC (1:2000) |
| Antibody | Anti-CtBP2 (mouse monoclonal) | BD Biosciences | Cat. #: 612044 RRID:AB_399431 | IHC (1:1000) |
| Antibody | Anti-S opsin (goat polyclonal) | Krzysztof Palczewski lab | Generated by Bethyl Laboratories | IHC (1:1000) |
| Antibody | Anti-M opsin (rabbit polyclonal) | Novus biologicals | Cat. #: NB110-74730 RRID:AB_1049390 | IHC (1:1000) |
| Antibody | Anti-ERK1/2 (rabbit monoclonal) | Cell Signaling Technology | Cat. #: 4695S RRID:AB_390779 | WB (1:2000) |
| Antibody | Anti-α-tubulin (rabbit polyclonal) | Cell Signaling Technology | Cat. #: 2144S RRID:AB_2210548 | WB (1:2000) |
| Antibody | Anti-phospho ERK1/2 (mouse monoclonal) | Cell Signaling Technology | Cat. #: 9106S RRID:AB_331768 | WB (1:1000) |
| Antibody | Donkey anti-rabbit AlexaFluor 647 secondary | Abcam | Cat. #: ab150075 RRID:AB_2752244 | IHC (1:500) |
| Antibody | Donkey anti-mouse AlexaFluor 488 secondary | Abcam | Cat. #: ab150105 RRID:AB_2732856 | IHC (1:500) |
| Antibody | Donkey anti-mouse AlexaFluor 555 secondary | Abcam | Cat. #: ab150106 RRID:AB_2857373 | IHC (1:500) |
| Antibody | Goat anti-guinea pig AlexaFluor 568 secondary | Abcam | Cat. #: ab175714 | IHC (1:500) |
| Antibody | Donkey anti-sheep AlexaFluor 488 secondary | Abcam | Cat. #: ab150177 RRID:AB_2801320 | IHC (1:500) |
| Antibody | Donkey anti-goat AlexaFluor 488 secondary | Abcam | Cat. #: ab150129 RRID:AB_2687506 | IHC (1:500) |
| Antibody | IRDye 800CW goat anti-rabbit | Licor | Cat. #: 926–32211 RRID:AB_621843 | WB (1:10000) |
| Antibody | IRDye 680RD donkey anti-mouse | Licor | Cat. #: 926–68072 RRID:AB_10953628 | WB (1:10000) |
| Software, algorithm | Keyence BZ-X800 Analyzer | Keyence Corporation of America | RRID:SCR_017205 | Microscopy image analysis |
| Software, algorithm | MetaMorph 7.8 | Molecular Devices | RRID:SCR_002368 | Microscopy image analysis |
| Software, algorithm | GraphPad Prism 8 | GraphPad Software Inc | RRID:SCR_002798 | Statistical analysis |
| Software, algorithm | OriginPro 2020b | OriginLab Inc | https://www.originlab.com/ | Analysis and graphing software |
| Chemical compound, drug | CBR-5884 | Sigma Aldrich | Cat. #: 1656 | Phosphoglycerate dehydrogenase inhibitor |
| Chemical compound, drug | DL-AP4 | Tocris Bioscience | Cat. #: 0101/100 | Glutamatergic antagonist |
| Chemical compound, drug | Picrotoxin; PTX | Sigma Aldrich | Cat. #: 528105 | GABA antagonist |
| Chemical compound, drug | Strychnine; STR | Sigma Aldrich | Cat. #: S0532 | Glycine antagonist |
| Chemical compound, drug | Meclofenamic acid; MFA | Sigma Aldrich | Cat. #: M4531 | Gap junction blocker |
| Chemical compound, drug | (1,2,5,6-Tetrahydropyridin-4-yl)methylphosphinic acid; TPMPA | Sigma Aldrich | Cat. #: T200 | GABAA-ρ antagonist |
| Chemical compound, drug | L-741626 | Tocris Bioscience | Cat. #:1003 | D2-antagonist |
| Chemical compound, drug | L-745870 | Tocris Bioscience | Cat. #:1002 | D4-antagonist |