Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | B6;129S6-Chattm2(cre)Lowl/J | Jackson Laboratory | Stock #: 006410 RRID:IMSR_JAX:006410 |
|
| Genetic reagent (M. musculus) | C57BL/6J | Jackson Laboratory | Stock #: 000664 RRID:IMSR_JAX:000664 |
|
| Genetic reagent (M. musculus) | Tg(Camk2a-cre)2Gsc | Günter Schütz, German Cancer Research Center | RRID:MGI:4457404 | Casanova et al., 2001; Wohleb et al., 2016 |
| Antibody | Anti-ChAT (goat polyclonal) | Millipore Sigma | Cat #: AB144P RRID:AB_2079751 |
(1:1000) |
| Antibody | Anti-GFP (chicken, polyclonal) | Thermo Fisher Scientific | Cat #: A10262, RRID:AB_2534023 | (1:1000) |
| Antibody | Anti-DsRed (rabbit, monoclonal) | Takara Bio | Cat #: 632392, RRID:AB_2801258 | (1:1000) |
| Antibody | Donkey anti-chicken 488 (secondary) | Jackson ImmunoResearch | Cat #: 703-545-155, RRID:AB_2340375 | (1:1000) |
| Antibody | Donkey anti-rabbit 555 (secondary) | Thermo Fisher Scientific | Cat #: A-31572, RRID:AB_162543 | (1:1000) |
| Antibody | Donkey anti-goat 555 (secondary) | Thermo Fisher Scientific | Cat #: A-21432, RRID:AB_141788 | (1:1000) |
| Antibody | Donkey anti-goat 647 (secondary) | Thermo Fisher Scientific | Cat #: A-21447, RRID:AB_141844 | (1:1000) |
| Recombinant DNA reagent | AAV9 hSyn-ACh3.0 | Yulong Li Jing et al., 2018;Jing et al., 2019 | Cat #: YL10002-AV9 | |
| Recombinant DNA reagent | AAV1 Syn-FLEX-GCaMP6s-WPRE-SV40 | Addgene | Cat #: 100845-AAV1 RRID:Addgene_100845 |
|
| Recombinant DNA reagent | AAV1-Syn-FLEX-jGCaMP7s-WPRE | Addgene | Cat #: 104491-AAV1 RRID:Addgene_104491 |
|
| Recombinant DNA reagent | AAV1 Syn-FLEX-NES-jRCaMP1b-WPRE-SV40 | Addgene | Cat #: 100850-AAV1 RRID:Addgene_100850 |
|
| Recombinant DNA reagent | AAV2 EF1a-DIO-EYFP | UNC Viral Vector Core | RRID:SCR_002448 | |
| Recombinant DNA reagent | AAV2 EF1a-DIO-hChR2(H134R)-EYFP | UNC Viral Vector Core | RRID:SCR_002448 | |
| Chemical compound, drug | Mecamylamine hydrochloride | Millipore Sigma | Cat #: M9020 | |
| Chemical compound, drug | (-) Scopolamine hydrochloride | Millipore Sigma | Cat #: S1013 | |
| Software, algorithm | MATLAB | MathWorks | RRID:SCR_001622 | Version 2020a |
| Software, algorithm | GraphPad Prism 8 | GraphPad Software | RRID:SCR_002798 | |
| Software, algorithm | EthoVision XT 10 | Noldus | RRID:SCR_000441 | |
| Software, algorithm | FV10-ASW | Olympus | RRID:SCR_014215 | Version 04.02.03.06 |
| Software, algorithm | Doric Neuroscience Studio | Doric Lenses | Version 5.3.3.14 | |
| Software, algorithm | MED-PC IV | Med Associates Inc | RRID:SCR_012156 | |
| Other | Allen Reference Atlas | Lein et al., 2007 | RRID:SCR_013286 | |
| Other | DAPI stain | Thermo Fisher Scientific | Cat #: 62248 | 1:1000 |