Figure 6. Minimal disruption of temporal modularity in UNC-3 function leads to locomotion defects.

(A) Schematics illustrating four cfi-1 alleles tested for behavioral analysis. (B–G) Examples of six locomotion features significantly disrupted in animals carrying a putative null (ot786) allele for cfi-1. Locomotion analysis was performed on day 2 adult worms. Animals lacking cfi-1 expression (initiation and maintenance) specifically in MNs (mNG::AID::cfi-1 ^{Δenhancer (769 bp)}) and animals unable to maintain cfi-1 expression in cholinergic MNs (mNG::AID::cfi-1^{8 COE motifs mut}) display locomotion defects when compared to control mNG::AID::cfi-1 animals. As expected, these defects are milder when compared to animals carrying the cfi-1 (ot786) allele. Panels B-F show locomotion features related to body curvature, whereas panel G shows radial velocity of the neck. A detailed description of each locomotion feature is provided below. (B) curvature_std_mid-body_w_backward_abs_10th: 10th percentile of the absolute value of the standard deviation of the curvature of the mid-body, while worm is moving backwards. (C) curvature_tail_w_backward_abs_90th: 90th percentile of the absolute value of the curvature of the tail, while worm is moving backwards. (D) curvature_std_mid-body_w_backward_50th: 50th percentile of the standard deviation of the curvature of the mid-body, while worm is moving backwards. (E) curvature_std_hips_abs_10th: 10th percentile of the absolute value of the standard deviation of the curvature of the hips. (F) curvature_std_hips_abs_50th: 50th percentile of the absolute value of the standard deviation of the curvature of the hips. (G) d_rel_to_body_radial_vel_neck_w_forward_90th: 90th percentile of the derivative of radial velocity of the neck relative to the centroid of the mid-body points, while worm is moving forwards.