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. 2020 Oct 1;24:17. doi: 10.1186/s40824-020-00196-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 11 — IHC analysis of Opn in calvarial defect of rat 4 and 8 weeks of post-implantation of the MEW PCL scaffolds. a) Blank (defect control without implanted scaffold), 250 μm, 500 μm. b) Offset.50.50, Grad.750top, Grad.250top. Opn: Osteopontin staining, Iso: Isotype control. The white bar represents 200 μm.c) Quantitative analysis at weeks 4 and 8. The values expressed are the mean ± SD (n = 3). Statistically significant differences between mean Opn intensity scores for the treatment groups when compared to the other groups were determined by ANOVA (Significant: * p < 0.0001, ^ p = 0.0004, ■ p < 0.0001, ♦ p = 0.0006, ▼p = 0.0004, ● p = 0.002)