Fig. 5. Expression of E2/E4/E5 is associated with differential activation of FGFR networks compared to E6/E7 expressing tumors.

Heatmaps of significant gene sets correlated with HPV gene subtypes depending on ssGSEA of TCGA HPV positive HNSCC data (a) and JHH HPV positive OPSCC data (b) were shown. Forty-two overlapped most differentially enriched gene sets were chosen in E2/E4/E5 subtype, while 41 overlapped most differentially enriched gene sets were chosen in E6/E7 subtype. Despite significant overlap, we noted significant enrichment for three FGFR network gene sets (labeled with red arrows) in a subset of tumors with E2/E4/E5 expression that were validated in a separate JHH HPV positive OPSCC dataset.