Fig. 3. SGK2 inhibition improves platinum response in platinum-resistant EOC, primary EOC, head and neck squamous cell carcinoma and breast cancer cells.

a Graph reporting cell viability of MDAH parental and PT-res cells treated with GSK650394 35 µM for 24 h (GSK), in the presence of increasing doses of CBDCA for additional 16 h. Results are expressed as survival ratio (%) between treated and untreated cells (set as 100% as reference). On the right, WB analysis reports the expression of SGK2. Vinculin was used as loading control. b SGK2 mRNA expression in the indicated primary and established EOC cell lines evaluated by qRT-PCR normalized to housekeeping gene POL2A and then folded on SKOV3 SGK2 expression. c Nonlinear regression analyses of cell viability assay in primary EOC cells treated with increasing doses of CDDP for 16 h. Results are expressed as percentage of viable cells respect to the untreated cells and represent the mean (±SD) of three biological replicates. The table on the right reports the IC50 and the confidence interval (C.I.) of each condition. Fisher’s exact test was used to calculate the global p value reported in the graph. d Graph reporting cell viability of MDA-MB-468, BT-549 (TNBC cell lines) and FaDu and CAL27 (HNSCC cell lines) treated with GSK650394 35 µM (GSK) and CBDCA as indicated. Results are expressed as survival ratio (%) between treated and untreated cells (set as 100% as reference). On the right, WB analysis reporting the expression of SGK2. Vinculin was used as loading control. (See also Fig. S4).