Fig. 1.

Analysis of the effects of test compounds on the growth of Cryptosporidium parvum in human ileocecal adenocarcinoma (HCT-8) cells in vitro. Equal amounts of freshly excysted sporozoites of C. parvum were inoculated into HCT-8 cells in culture and immediately treated with 20 μM of compound JS-1-07, JS-1-09, JS-1-11, JS-1-37, JS-2-20, JS-2-35, JS-2-49, GMG-1-08 or GMG-1-09. Infected cells without compound-treatment, but with equivalent amount of DMSO to that used in compound-treated cells (1% v/v) were set as negative controls. Positive control cultures were treated with paromomycin (800 μM). After 72 h the cultures were analyzed for parasite proliferation by immunofluorescence assays. The fluorescence generated by C. parvum was quantified and is shown on the Y-axis representing the parasite load. The data shown represent the means from three independent experiments with standard error bars, and levels of statistical significance depicted by asterisks (*, P < 0.05).