Fig. 2.

Analysis of the effects of test compounds on the growth of T. gondii in vitro. Equal amounts (500) of T. gondii tachyzoites constitutively expressing yellow fluorescent protein (YFP) were used to infect confluent monolayers of human foreskin fibroblasts (HFF) and immediately treated with 20 μM of compound JS-1-07, JS-1-09, JS-1-11, JS-1-37, JS-2-20, JS-2-35, JS-2-49, GMG-1-08 or GMG-1-09. Infected cells without compound-treatment, but with DMSO equivalent to volume used in compound-treated cells (1% v/v) were set as negative controls. Positive control cultures were treated with atovaquone (0.5 μM). After 48 h, the cultures were analyzed for parasite proliferation by measuring the parasite YFP fluorescence by fluorescence microscopy. The fluorescence generated by T. gondii was quantified and is shown on the Y-axis representing the parasite load. The data shown represent the means from three independent experiments with standard error bars, and levels of statistical significance depicted by asterisks (*, P < 0.05).